MMT-060562

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90111911, mouse breast, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse breast
• Conditionnement: tube of 5 μg 90111911-DNA-5UG; pkg of vial of cells 90111911-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 40; diploid
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse C57BL x A/F1 mammary tumour

Description de la lignée cellulaire

Derived from a spontaneous mammary tumour which arose in a C57BL x A/F1 hybrid female mouse. Bittner virus has been revealed in the cells by electron microscopy. Tumourigenic in BALB/c mice.

Application

Tumourigenicity and virus studies: Bittner virus

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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