45.6.TG1.7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse blood
• Conditionnement: tube of 5 μg 90110506-DNA-5UG; pkg of vial of cells 90110506-1VL
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblast
• Produits: Immunoglobulin G (IgG2b) ( kappa)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse BALB/c myeloma

Description de la lignée cellulaire

Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutated during growth in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.

Application

Fusion partner, immunoglobulin production

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 4-5x100,000 cells/ml; 5% CO₂; 37°C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.

Autres remarques

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