HT 1197

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87032403, human bladder, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human bladder
• Conditionnement: tube of 5 μg 87032403-DNA-5UG; pkg of vial of cells 87032403-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 46
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian bladder carcinoma

Description de la lignée cellulaire

Derived from a grade 4 transitional cell carcinoma of the bladder of a 44 year old Caucasian male who had not received chemo or radiation therapy. The cells grow in soft agar, produce fibrinolytic activity and are tumorigenic in mice and hamsters.

Application

HT 1197 has been used to study the transcription activity of human endogenous retroviruses (HERV).

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 16,18

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2x10,000 to 5x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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