P815-1-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse breast
• Conditionnement: tube of 5 μg 85011406-DNA-5UG; pkg of vial of cells 85011406-1VL
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse DBA/2 mastocytoma

Description de la lignée cellulaire

From DBA/2 mouse tumour treated with methylcholanthrene. Used as target cell for cytotoxic T-cell assays. Cells exhibit no effector activity in antibody dependent cell mediated cytotoxic system. Growth not inhibited by dextran sulphate, LPS or PPD.

Application

Cytotoxicity and tumourigenicity studies

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml. The cells are non-adherent but can give rise to adherent cells with large granules; 5% CO₂; 37°C.

Autres remarques

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