LA1-55n

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human nerve
• Conditionnement: tube of 5 μg 06041203-DNA-5UG; pkg of vial of cells 06041203-1VL
• Mode de croissance: Semi-adherent aggregates
• Caryotype: Not specified
• Morphologie: Fusiform cells
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Neuroblastoma

Description de la lignée cellulaire

Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma.LA-N-1 (Sigma Catalogue number 06041201), LA1-55n (Sigma Catalogue number 06041203) and LA1-5s (Sigma Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Milieu de culture

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 to 1:100 ratio; 8% CO₂, 37°C. Cells are moderate to weakly adherent. Allow floating clumps to settle then withdraw medium. Remove at

Autres remarques

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