RK-13 Cell Line from rabbit

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21715, rabbit kidney, Epithelial-like

• Synonyme(s) : GL RK/13 Cells, Glaxo Laboratories Rabbit Kidney 13 Cells, R13 Cells
• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• Source biologique: rabbit kidney
• Conditionnement: tube of 5 μg 00021715-DNA-5UG; pkg of vial of cells 00021715-1VL
• Mode de croissance: Adherent
• Caryotype: 2n=44, stem-line = 56 (=22%)
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rabbit kidney

Description de la lignée cellulaire

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

Application

Virus studies

Milieu de culture

EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

Autres remarques

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