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C3228

Sigma-Aldrich

CelLytic™ MT Cell Lysis Reagent

For mammalian tissues

Synonyme(s) :

Cell lysis buffer, Cell lysis reagent

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About This Item

Code UNSPSC :
41116134
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Forme

solution

Catégories apparentées

Application

CelLytic™ MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Caractéristiques et avantages

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Autres remarques

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Qualité

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Informations légales

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
099M4092V
11190414
059M4132V
059M4131V
11181119

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M Espe et al.
SpringerPlus, 4, 658-658 (2015-11-07)
Hydrolyzed fish proteins (H-pro) contains high concentrations of free amino acids and low molecular peptides that potentially benefit health. The following study aimed to test whether the water soluble phase of H-pro could reduce apoptosis and inflammation in primary liver
Resveratrol Decreases Oxidative Stress by Restoring Mitophagy and Improves the Pathophysiology of Dystrophin-Deficient mdx Mice
Sebori R, et al.
Oxidative Medicine and Cellular Longevity, 2018 (2018)
Leticia E Camacho et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(2), R101-R109 (2017-05-12)
Intrauterine growth restriction (IUGR) is associated with persistent metabolic complications, but information is limited for IUGR infants. We determined glucose-stimulated insulin secretion (GSIS) and insulin sensitivity in young lambs with placental insufficiency-induced IUGR. Lambs with hyperthermia-induced IUGR (
Quantitative expression and localization of GABAB receptor protein subunits in hippocampi from patients with refractory temporal lobe epilepsy
Sheilabi MA, et al.
Neuropharmacology, 136, 117128-117128 (2018)
Joseph S Marino et al.
The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a
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