A3422
Achromopeptidase from Lysobacter enzymogenes
partially purified powder, ≥20,000 units/mg solid
Synonyme(s) :
ACP, Achromopeptidase
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About This Item
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Forme
partially purified powder
Niveau de qualité
Activité spécifique
≥20,000 units/mg solid
Poids mol.
27 kDa
Activité étrangère
Collagenase, present
Température de stockage
−20°C
Application
Achromopeptidase from bacteria may be used:
- in the homogenization step for genomic DNA extraction from microbial biofilm
- in the lysis of gram-positive strains(
- for DNA extraction from Nocardia strains
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Actions biochimiques/physiologiques
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Définition de l'unité
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
Forme physique
Partially purified powder.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocoles
Enzymatic Assay of Achromopeptidase
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