11271164001

Interleukin-2, mouse (mIL-2)

recombinant (E. coli)

• Synonyme(s) : mIL-2, mouse interleukin-2
• Code UNSPSC : 12352207

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Essai: >95% (SDS-PAGE)
• Forme: solution
• Puissance: <0.5 ng/mL EC₅₀
• Poids mol.: 17,200 Da
• Conditionnement: pkg of 10,000 U (5 μg, 1 ml)
• Fabricant/nom de marque: Roche
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): inhibition assay: suitable
• Impuretés: <0.1 EU/μgtested (LAL test)
• Numéro d'accès UniProt: P04351
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Il2(16183)

Description

Description générale

Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane

Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).

Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.

Spécificité

Mouse IL-2 is effective on mouse cells, but not on human cells.

Application

Recombinant murine IL-2 is produced in E. coli and exhibits the following:

• Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
• Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
• Weakly inhibits the binding to human responder cells.
• Shares identical biological and immunological activities with human IL-2.
• Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
• used for the stimulation of murine T-cells

Qualité

Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Séquence

Chain Length 149 AA

One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.

Définition de l'unité

EC₅₀ definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).

Notes préparatoires

Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.

Autres remarques

Specific activity/EC ₅₀: >2 x 10⁶ U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1^st international standard, 86/504), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC ₅₀ definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available