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SCT052

Sigma-Aldrich

BioTracker Far-Red Peroxynitrite Live Cell Dye

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About This Item

Code UNSPSC :
12171501
Nomenclature NACRES :
NA.32

Type

for cell culture

Technique(s)

cell based assay: suitable

Méthode de détection

fluorometric

Description générale

Reactive oxygen species (ROS) and nitrogen species (RNS) are implicated in various physiological and pathological processes. Among ROS and RNS, peroxynitrite (ONOO−) has attracted much special attention because of the key roles that it plays in signal transduction and antimicrobial activities. Peroxynitrite is endogenously produced by reaction of nitric oxide (NO) and superoxide radical anion (O2), however, ONOO− is more cytotoxic than NO or O2 alone, contributing to numerous pathologies such as cardiovascular disease and injury, diabetes, and cancer.

The BioTracker Far-Red Peroxynitrite live cell dye can be used for ONOO- detection. This probe is cell permeable and displays a high sensitivity and selectivity towards peroxynitrite over other ROS/RNS.
This probe can be used to detect exogenous and endogenous peroxynitrite in living systems and displays long wavelength absorption (600 nm) and emission (638 nm) bands.

Spectral Properties
Absorption: 600 nm
Emissions: 638 nm

References
Wu D et al. A Far-Red-Emitting Fluorescence Probe for Sensitive and Selective Detection of Peroxynitrite in Live Cells and Tissues. Anal Chem. 2017, 89, 10924-10931.

Application

Live cell fluorescent imaging

Qualité

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 480.60 g/mol

Propriétés physiques

480.60 g/mol

Forme physique

Lyophilized

Stockage et stabilité

Store BioTracker Far red Peroxynitrite Live Cell Dye at -20°C, desiccate and protect from light Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Informations légales

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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