SCC652

GLUTag Mouse Colonic Endocrine Cell Line

• Synonyme(s) : GLUTag, GLUTag cells, Murine GLUTag cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conditionnement: vial of ≥1 x 10^6 vial (viable cells per vial)
• Fabricant/nom de marque: Millipore
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: liquid nitrogen
• Température de stockage: −196°C

Description

Application

• Each vial contains > 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Caractéristiques et avantages

GLUTag cells are murine intestinal endocrine L-cells derived from colonic tumors. They secrete a variety of pro-glucagon derived peptides (PGDPs) such as GLI, IRG, and GLP-1. GLUTag cells are among one of the most widely used cell models to understand GLP-1 secretion.

Description de la cible

Glucagon-like peptide-1 (GLP-1), an important regulator of glucose dependent insulin secretion, is a peptide hormone produced by enteroendocrine cells.² It also plays a role in food intake and gut motility. GLP-1 activity is preserved in individuals with type 2 diabetes, allowing it to be an effective target for drugs to treat diabetes and obesity.

The GLUTag cell line is an effective intestinal endocrine L-cell model derived from colonic tumors of transgenic mice. This cell line secretes a variety of pro-glucagon derived peptides (PGDPs) such as GLI, IRG, and GLP-1.¹ GLUTag cells are among one of the most widely used cell models to understand GLP-1 secretion. GLUTag cells are capable of activating GLP-1 secretion in vivo and have a phenotype that closely mirrors non-immortalized intestinal L-cells with some slight genetic differences. The immunoreactive profile of GLUTag cells also suggests L-cell lineage by being positively immunoreactive to GLP-1 and cholecystokinin, but negative for a large variety of other hormones such as calcitonin, insulin, and gastrin.

Although the GLUTag cell line has murine origins, they have been shown to have a relevant response mechanism that essentially mirrors the GLP-1 secretion response in humans. This means that testing potential therapeutic agents using GLUTag cells has been shown to still have a strong relevance to a human model. The ease-of-use of GLUTag cells becomes quite invaluable when compared to the difficulties of using primary human L-cells.

Source
Derived from an endocrine carcinoma in a mouse expressing SV40 Large T antigen. Fragments of the tumors were subcutaneously propagated in nude mice and individual cells from one of the tumors was isolated and single-cell cloned.

References
1. Drucker DJ, Jin T, Sylvia L, Young TA, Brubaker PL. 1994. Activation of proglucagon gene transcription by protein kinase-A in a novel mouse enteroendocrine cell line. Molecular Endocrinology. 8(12):1646–1655.
2. Kuhre RE, Wewer Albrechtsen NJ, Deacon CF, Balk-Møller E, Rehfeld JF, Reimann F, Gribble FM, Holst JJ. 2016. Peptide production and secretion in GLUTag, NCI-H716, and STC-1 cells: a comparison to native L-cells. Journal of Molecular Endocrinology. 56(3):201–211.

Stockage et stabilité

GLUTag cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

Autres remarques

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable