SCC064SF

LX-2 Hepatic Stellate Cell Line-SF Adapted

• Synonyme(s) : HSC, Hepatic lipocytes, Hepatic pericytes, Ito cells, Perisinusoidal cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Conditionnement: vial of ≥1 x 10^6 vial (viable cells per vial)
• Fabricant/nom de marque: Millipore
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: liquid nitrogen
• Température de stockage: −196°C

Description

Application

• Each vial contains > 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases tested by using the Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Caractéristiques et avantages

Serum-Free media adapted LX-2 human hepatic stellate cells that been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making them a highly suitable model of human hepatic fibrosis.

Description de la cible

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

The LX-2 Hepatic Stellate Cell Line-SF Adapted cell ine (SCC064SF) has been preadapted to serum-free media conditions using the LX-2 Human Hepatic Stellate Serum-Free Media (SCM064SFM). It is recommended to use SCM064SFM to thaw and expand the cell line.

Stockage et stabilité

LX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.

Autres remarques

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable