PS1023
PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)
liquid, clone C25A9, Calbiochem®
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
C25A9, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
human
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Isotype
IgG
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
phosphorylation (pSer423/pSer425)
Informations sur le gène
human ... SMAD3(4088)
Description générale
Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425.
Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
This PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer423/425).
Immunogène
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.
Application
Immunoblotting (1:1000)
Immunoprecipitation (1:50)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.
Autres remarques
Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Amy M Gancarz et al.
Nature neuroscience, 18(7), 959-961 (2015-06-02)
Activin receptor signaling, including the transcription factor Smad3, was upregulated in the rat nucleus accumbens (NAc) shell following withdrawal from cocaine. Direct genetic and pharmacological manipulations of this pathway bidirectionally altered cocaine seeking while governing morphological plasticity in NAc neurons.
Javier Milara et al.
Mucosal immunology, 14(2), 377-388 (2020-09-06)
Several mucins are implicated in idiopathic pulmonary fibrosis (IPF); however, there is no evidence regarding the role of MUC4 in the development of IPF. Here we demonstrated that MUC4 was overexpressed in IPF patients (n = 22) compared with healthy subjects (n = 21)
Beatriz Ballester et al.
International journal of molecular sciences, 22(12) (2021-07-03)
Several transmembrane mucins have demonstrated that they contribute intracellularly to induce fibrotic processes. The extracellular domain of MUC16 is considered as a biomarker for disease progression and death in IPF patients. However, there is no evidence regarding the signalling capabilities
Alfonso Pastor-Clerigues et al.
PloS one, 9(12), e115404-e115404 (2014-12-17)
Home parenteral nutrition (PN) is associated with many complications including severe hepatobiliary dysfunction. Commercial ω-6 fatty acid-soybean based-lipid emulsions in PN may mediate long term PN associate liver disease (PNALD) whereas ω-3-fish oil parenteral emulsions have shown to reverse PNALD
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