MABS2002

Anti-mPEG, clone 15-2b

• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: monoclonal
• Poids mol.: (N/A)
• Produit purifié par: using Protein A
• Espèces réactives: mouse,
• Isotype: IgG2b, kappa

Description

Spécificité

Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).

Application

Anti-mPEG, clone 15-2b, Cat. No. MABS2002, is a mouse monoclonal antibody that detects methoxy-PEG (mPEG)-conjugated proteins and has been tested for use in ELISA.

ELISA

Caractéristiques et avantages

Methoxy PEG covalently conjugated to E.coli beta-glucuronidase.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.

Autres remarques

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solutions. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. The monomethoxylated form of polyethylene glycol (mPEG) has been widely employed in drug modifications. mPEG modification reduces nonspecific binding and prevents recognition by the reticuloendothelial system. This monoclonal antibody (clone 15-2b) specifically binds to the terminal methoxy group, but not the backbone of PEG. It displays high affinity for the terminal methoxy group and can be easily used as a detection antibody for the quantification of mPEG by ELISA. It is also suitable for detection of PEGylated liposomes and nanoparticles. The mPEG cell-based competition ELISA can detect lower molecular-weight (less than 2 kDa) mPEG molecules even at nanomolar concentration and the assay is not affected by the presence of mouse or human serum. In addition, mPEG cell-based ELISA can be useful in determining the pharmacokinetics of a mPEG molecule an animal without the need for any radiolabeling

Evaluated by ELISA with methoxy Polyethylene Glycol (10KPEG, 3KPEG, and 2K mPEG).

ELISA Analysis: 5 µg/mL of this antibody detected methoxy polyethylene glycol (2K-mPEG) and with minimal reactivity with 10K and 3K amino-PEGs.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.