MABN2421

Anti-Beta (β)-Amyloid antibody

mouse monoclonal, Mhj7.4

• Synonyme(s) : Ab42, Amyloid peptide 1-42
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Nom du produit: Anti-Amyloid β 1-42 Antibody, clone mHJ7.4, clone mHJ7.4
• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Clone: mHJ7.4, monoclonal
• Poids mol.: 4.51 kDa (for Amyloid 1-42 peptide; 86.94 kDa calculated for Amyloid Precursor Protein.)
• Produit purifié par: using protein G
• Espèces réactives: human
• Isotype: IgG2bκ
• Numéro d'accès: NP_000475
• Numéro d'accès UniProt: P05067

Description

Description générale

Amyloid-beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. Deposition of Aβ peptides is an early event in the pathogenesis of Alzheimer s disease (AD) that precedes the formation of Tau-positive paired helical filaments (PHFs) in NFTs. AD is also characterized by a progressive deposition of the Aβ peptide in senile plaques. Aβ peptides originate from the proteolytic cleavage of the amyloid precursor protein (APP).

Processing of APP occurs by two major pathways cleavage of APP by a-secretase is a non-amyloidogenic pathway and does not produce Aβ peptides. Cleavage of APP at the N-terminus of the Aβ region by β-secretase and at the C-terminus by g-secretase represents the amyloidogenic pathway. The β-secretase cleaves APP between residues Met671 and Asp672 and yields sAPPβ and C99. Following the β-secretase cleavage, a second cleavage occurs at the C-terminus of Aβ peptide that releases Aβ from C99. This cleavage occurs in the vicinity of residue 712 of the C-terminus. The g-secretase can cleave the C-terminal region at either Val711 or Ile713 to produce the shorter Aβ peptide (Aβ1-40) or the longer Aβ peptide (Aβ1-42), respectively.

The predominant form of Aβ found in the cerebrospinal fluid is the shorter Aβ1-40 peptide. Despite its lower rate of synthesis, Aβ1-42 is the peptide that is initially deposited within the extracellular plaques of AD patients. In addition, Aβ1-42 is more hydrophobic and aggregates at much lower concentration than the Aβ1-40 form. The abnormal accumulation of Aβ peptides can result in neuronal damage and loss by increasing free radical production and activation of inflammatory pathways by enhancing microglial secretion of inflammatory cytokines. Interaction between Aβ and ApoE3 or E4 is also an important determinant of amyloidosis. ApoE3 is shown to inhibit Aβ aggregation in vitro by decreasing Aβ multimers, whereas ApoE4 is reported to accelerate the rate of amyloid fibril formation.

Spécificité

Clone mHJ7.4 is a mouse monoclonal antibody that detects Amyloid β 1-42 peptide. It targets an epitope within 6 amino acids from the C-terminal region of the peptide.

Immunogène

A linear peptide corresponding to six amino acids (37-42) from the C-terminal region of human Amyloid β1-42 peptide.

Application

ELISA Analysis: A representative lot detected Amyloid β 1-42 in ELISA applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Qualité

Evaluated by ELISA using human Amyloid β 1- 42 peptide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Stable for 1 year at +2°C to +8°C from date of receipt.

Autres remarques

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable