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DC01L

Sigma-Aldrich

Goat Anti-Rat IgG Peroxidase Conjugate

lyophilized, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.54

Source biologique

goat

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

lyophilized

Ne contient pas

preservative

Espèces réactives

rat

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Isotype

IgG

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoaffinity purified goat polyclonal antibody adsorbed against human, bovine, and horse serum proteins and conjugated to horseradish peroxidase. Recognizes rat IgG.
This Goat Anti-Rat IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rat IgG.

Immunogène

Rat

Application

ELISA (1:5000-1:100,000)

Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)

Immunohistochemistry (1:500-1:5000)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.

Reconstitution

Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.

Autres remarques

To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rat IgG and light chains common to most rat immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human, bovine, and horse serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

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Sina Wittmann et al.
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