ABE2586

Anti-NR2F2 (ARP-1) isoform B

from rabbit

• Synonyme(s) : COUP transcription factor 2, COUP-TF2, Apolipoprotein A-I regulatory protein 1, ARP-1, COUP transcription factor II, COUP-TF II, Nuclear receptor subfamily 2 group F member 2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: rat
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology), sheep (based on 100% sequence homology), human (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_001138627
• Numéro d'accès UniProt: P24468-2
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NR2F2(7026)

Description

Description générale

COUP transcription factor 2 (UniProt: P24468-2; also known as COUP-TF2, COUP-TF II, Apolipoprotein A-I regulatory protein 1, ARP-1, COUP transcription factor II, COUP-TF II, Nuclear receptor subfamily 2 group F member 2) is encoded by the NR2F2 (also known as ARP1, TFCOUP2) gene (Gene ID: 7026) in human. Coup-TF II is a ligand-activated transcription factor that is activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid. Its DNA binding region is localized in amino acids 76-151. COUP-TF II contains two NR C4-type zinc finger domains (aa 79-99 and 115-139). It is widely distributed in human tissues with detectable expression in all tissues examined. Its tissue and cell-specific expression can be modulated both transcriptionally and post-translationally. Through binding to 5 -AGGTCA-3 direct repeats (DRs) with variable spacing, it can modulate the expression of target genes, such as RA receptor beta 2, PEPCK, NGFI-A, and cholesterol 7 -hydroxylase. COUP-TF II action can also be enhanced by interaction with certain coactivators, such as with members of the steroid receptor coactivator family members SRC-1, SRC-2, and SRC-3. Mutations in NR2F2 gene have been linked to multiple types of congenital heart defects characterized transposition of the great arteries, aortic stenosis, and ventricular septal defect. (Ref.: Litchfield, LM and Klinge, CM (2012). J. Mol. Endocrinol. 49 (3); R135-R148).

Spécificité

This rabbit polyclonal antibody detects COUP-TF II. It targets an epitope within 14 amino acids from the N-terminus region of isoform B.

Immunogène

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the first 14 amino acids from the N-terminus region of human COUP transcription factor 2 (ARP1), isoform B.

Application

Anti-NR2F2 isoform B, Cat. No. ABE2586, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 2 group F member 2. and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected NR2F2 isoform B in in 10 µg of L6 cell lysate.

Western Blotting Analysis: A representative lot detected NR2F2 isoform B in MEWO cells overexpressing NR2F2 isoform B.(Courtesy of Claudia Lovell at Hernando Lab, NYU Langone Medical Center).

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected NR2F2 isoform B in human kidney, human pancreas, and human cerebellum tissues.

Qualité

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NR2F2 isoform B in in 10 µg of PC12 cell lysate.

Description de la cible

~37kDa observed; 31.48 kDa calculated for isoform B. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1