ABC936

Anti-RINT-1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : RAD50-interacting protein 1, HsRINT-1, RINT-1, RAD50 interactor 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_068749
• Numéro d'accès UniProt: Q6NUQ1
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RINT1(60561)

Description

Description générale

RAD50-interacting protein 1 (UniProt Q6NUQ1; also known as HsRINT-1, RINT-1, RAD50 interactor 1) is encoded by the RINT1 gene (Gene ID 60561) in human. Originally identified and named as a RAD50-interacting partner by a yeast two-hybrid screening, RINT1 is involved in the G2/M checkpoint, maintenance of centrosome integrity, telomere elongation, Golgi apparatus, Golgi-ER vesicular trafficking and Cis- and Trans-Golgi homeostasis. Rint1 homozygous deletion in mice causes early embryonic lethality, while Rint1 heterozygotes are predisposed to multiple tumor formation. Likewise, rare RINT1 mutations are identified to be associated with intermediate levels of breast cancer risk in human.

Spécificité

This polyclonal antibody detects RINT-1 by targeting an N-terminal region sequence.

Immunogène

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding to an N-terminal sequence of human RINT-1.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected RINT-1 in A431, HeLa, HepG2, and NIH/3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-RINT-1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of RINT-1.

Qualité

Evaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RINT-1 in 10 µg of MOLT-4 cell lysate.

Description de la cible

~85 kDa observed. 90.63 kDa calculated. Target band may be resolved into a doublet with the slower migrating band representing product resulting from translation starting at an non-AUG codon (Xiao, J., et al. (2001). J. Biol. Chem. 276(9):6105-6111). Uncharacterized band(s) may appear in some lysates.

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1