AB9754-I

Anti-Kisspeptin-10

• Synonyme(s) : KISS1, Kisspeptin-1, Kisspeptin-10, Metastasis-suppressor KISS-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Niveau de qualité: 200
• Source biologique: rabbit
• Forme d'anticorps: purified antibody
• Clone: polyclonal
• Poids mol.: ~16 kDa
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Isotype: IgG
• Numéro d'accès: NP_002247
• Numéro d'accès UniProt: Q15726

Description

Description générale

Metastasis-suppressor KISS-1 (UniProt: Q15726; also known as Kisspeptin-1) is encoded by the KISS1 (also known as PP5098) gene (Gene ID: 3814) in human. KISS1 is highly expressed in placenta and some expression is also observed in testis, pancreas, liver, small intestine. Low expression levels are also observed in the brain. Its expression is shown to increase in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Its expression is reported to be higher levels in first trimester trophoblasts than at term of gestation. KISS-1 is a secreted metastasis suppressor that demonstrates inhibition of metastases formation in several types of cancers. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that can also be cleaved into four chains known as Metastin (aa 68-121), Kisspeptin-14 (aa 108-121), Kisspeptin-13 (aa 109-12), and Kisspeptin-10 (112-121). Metastin acts as an endogenous ligand of GPR54, a GPCR. Activation of this receptor inhibits cell proliferation and cell migration that contributes to its tumor suppressor role. Kisspeptin-10 is reported to be a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself and increases intracellular Ca2+ levels in isolated first trimester trophoblasts. KISS1 expression is reported to be significantly reduced in human pancreatic cancer tissue and this reduced expression is associated with advanced disease. KISS1 also diminishes MMP9 expression by effecting reduced NF-kB binding to the promoter.

Spécificité

This rabbit polyclonal antibody detects Kisspeptin. It targets an epitope within Kisspeptin-10 at the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region (Kisspeptin-10).

Application

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Kisspeptin-10 in human placenta tissue sections

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Qualité

Evaluated by Western Blotting in HL-60 cell lysate.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Stable for 1 year at +2°C to +8°C from date of receipt.

Autres remarques

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable