AB3150
Anti-β-Actin (ACTB) Antibody
CHEMICON®, rabbit polyclonal
Synonyme(s) :
32 kDa Fragment of beta-Actin
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Nom du produit
Anti-Fractin Antibody, CT, serum, Chemicon®
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human, rat, mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Description générale
During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.
Spécificité
By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin.
Immunogène
K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin.
Application
Detect Fractin using this Anti-Fractin Antibody, C-terminus validated for use in WB, IC, IH(P).
Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
Forme physique
Liquid whole rabbit serum containing no preservatives.
Stockage et stabilité
Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
Remarque sur l'analyse
Control
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Distinct nuclear and cytoplasmic localization of caspase cleavage products in two models of induced apoptotic death in dopamine neurons of the substantia nigra.
Oo, Tinmarla F, et al.
Experimental neurology, 175, 1-9 (2002)
Fractin immunostaining for the detection of apoptotic cells and apoptotic bodies in formalin-fixed and paraffin-embedded tissue.
A J Suurmeijer et al.
Laboratory investigation; a journal of technical methods and pathology, 79(5), 619-620 (1999-05-20)
Zhouheng Ye et al.
Journal of neuropathology and experimental neurology, 77(3), 260-265 (2018-02-07)
Our previous study demonstrated caspase independent DNA fragmentation after very brief cerebral ischemia, the mechanism of which was unclear. In this study, we explore whether actin is cleaved following focal cerebral ischemia, and whether these structural changes of actin might
B Elibol et al.
Neuroscience, 105(1), 79-86 (2001-08-03)
Nitric oxide can promote or inhibit apoptosis depending on the cell type and coexisting metabolic or experimental conditions. We examined the impact of nitric oxide on development of apoptosis 6, 24, and 72 h after permanent middle cerebral artery occlusion
F Yang et al.
The American journal of pathology, 152(2), 379-389 (1998-02-18)
During apoptosis, activation of a family of cysteine proteases related to interleukin-1beta-converting enzyme (ICE)-related proteases or "caspases" results in endoproteolytic cleavage of multiple substrates at specific aspartate residues. We have sought to develop new antibody probes for the neoepitopes in
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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