444203

2-mercaptoéthanol

A disulfide-reducing agent.

• Synonyme(s) : β-mercaptoéthanol, 2-hydroxyéthylmercaptan, BME, Thioéthylèneglycol
• Formule linéaire : HSCH₂CH₂OH
• Numéro CAS: 60-24-2
• Poids moléculaire : 78.13
• Beilstein: 773648
• Numéro MDL: MFCD00004890
• Code UNSPSC : 12161504
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 100
• Densité de vapeur: 2.69 (vs air)
• Pression de vapeur: 1 mmHg ( 20 °C)
• Description: Merck USA index - 14, 5869
• Essai: ≥99% (GC)
• Forme: liquid
• Limite d'explosivité: 18 %
• Pertinence de la réaction: reagent type: reductant
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Concentration: 14.3 M (pure liquid)
• Indice de réfraction: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• pb: 157 °C (lit.)
• Pf: <-50 °C
• Densité: 1.12 g/cm³ at 20 °C; 1.114 g/mL at 25 °C (lit.)
• Traces de cations: heavy metals (as Pb): ≤0.0001%
• Absorption: ≤0.31 at 280 nm; ≤1.50 at 260 nm
• Activité étrangère: DNases, proteases, RNases, none detected
• Conditions d'expédition: ambient
• Température de stockage: 15-25°C
• Chaîne SMILES: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• Clé InChI: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

Description générale

A disulfide-reducing agent.

A disulfide-reducing agent. Reagent commonly used in organic synthesis (aborsorbance: ≤1.50 at 260 nm; ≤0.31 at 280 nm).

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Avertissement

Toxicity: Toxic (F)

Autres remarques

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Conseils de prudence: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Classification des risques: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Organes cibles: Liver,Heart
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 165.2 °F - closed cup
• Point d'éclair (°C): 74 °C - closed cup