T9281

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, suitable for electrophoresis, ≥99.0%

• Synonym(s): 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
• Linear Formula: (CH₃)₂NCH₂CH₂N(CH₃)₂
• CAS Number: 110-18-9
• Molecular Weight: 116.20
• Beilstein: 1732991
• EC Number: 203-744-6
• MDL number: MFCD00008335
• UNSPSC Code: 41105319
• PubChem Substance ID: 24900591
• NACRES: NB.22

Properties

• product line: BioReagent
• Quality Level: 200
• Assay: ≥99.0%
• expl. lim.: 9.08 %
• technique(s): electrophoresis: suitable
• refractive index: n20/D 1.4179 (lit.)
• bp: 120-122 °C (lit.)
• mp: −55 °C (lit.)
• density: 0.775 g/mL at 20 °C (lit.)
• suitability: suitable for electrophoresis
• SMILES string: CN(C)CCN(C)C
• InChI: 1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3
• InChI key: KWYHDKDOAIKMQN-UHFFFAOYSA-N

Description

General description

TEMED (N,N,N′,N′-Tetramethylethylenediamine) is molecule which allows rapid polymerization of polyacrylamide gels. In the presence of ammonium persulfate, TEMED is responsible for the formation of free radicals from persulfate, thereby initiating the acrylamide polymerization process. However, excess concentration of TEMED can result in abnormal separation patterns.

Application

TEMED (N,N,N′,N′-Tetramethylethylenediamine) has been used for the preparation of acrylamide hydrogels and sodium dodecyl sulfate-polyacrylamide gels.

Caution

Handle and store under inert gas, air and moisture sensitive.

Analysis Note

Tested as a catalyst for PAGE.

Safety Information

• Pictograms: GHS02,GHS06,GHS05
• Signal Word: Danger
• Hazard Statements: H225,H302,H314,H331
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 61.7 °F - closed cup
• Flash Point(C): 16.5 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves