SEQXE

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

• Synonym(s): SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit
• UNSPSC Code: 41121800
• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): whole genome amplification: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

SeqPlex^™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina^®, SOLiD^™, or 454 sequencing workflows.

Application

SeqPlex^™ DNA Amplification Kit has been used for whole genome amplification.

Features and Benefits

• Random priming technology amplifies fragmented DNA such as ChIP or FFPE
• Facilitates sequencing from as little as 100 pg of ChIP DNA
• Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
• Compatible with Illumina^®, SOLiD^™, or 454 library prep for next generation sequencing

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.

Legal Information

Illumina is a registered trademark of Illumina, Inc.

SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• W4502 Water, Nuclease-Free Water, for Molecular Biology
• Library Preparation Buffer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable