ZRB3134

Anti-TCF7/TCF1 Antibody, clone 1H9 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): ,TCF-7, T-cell-specific transcription factor 1, TCF-1, Transcription factor 7
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1H9, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized

Description

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1H9 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects TCF7/TCF1. It targets an epitope within 25 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human TCF7/TCF1.

Application

Quality Control Testing

Evaluated by Western Blotting in EL4 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TCF7/TCF1 in EL4 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TCF7/TCF1 in MOLT-4 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound with TCF7/TCF1 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Flow Cytometry Analysis: 1 µg from a representative lot detected TCF7/TCF1 in one million MOLT4 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TCF7/TCF1 in human tonsil and human appendix tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Transcription factor 7 (UniProt: P36402; also known as TCF-7, T-cell-specific transcription factor 1 (T-cell factor 1; TCF-1)) is encoded by the TCF7 (also known as TCF1) gene (Gene ID: 6932) in human. Transcription Factor 7 (TCF7), also known as T-cell factor 1 (TCF-1), is a member of the TCF/LEF family of transcription factors. It contains a High Mobility Group (HMG) box domain that facilitates DNA binding and a β-catenin binding domain. Multiple TCF7 isoforms can be categorized into two distinct groups; the full-length isoforms (FL-TCF7) act as transcription activators, and the dominant negative isoforms (dn-TCF7), lacking the N-terminal β-catenin binding domain, as transcription repressors. TCF7 is involved in various signaling pathways, such as Wnt, FoxO1, Notch, and P21. In the Wnt signaling pathway, TCF7 acts as a transcriptional regulator. When Wnt signaling is activated, TCF7 partners with β-catenin to regulate the expression of target genes involved in T cell development and differentiation and embryonic development. In cancer, dysregulation of TCF7 and the Wnt pathway can lead to uncontrolled cell proliferation and tumorigenesis. TCF7 is also involved in autoimmune and lung diseases. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref: Zhu, Y., et al., (2015), J Transl Med 13, 273)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.