ZRB2528

Anti-BubR1 Antibody, clone 1D23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Mitotic checkpoint kinase MAD3L
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D23, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 119.55 kDa; observed mol wt ~120 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_001202.5
• UniProt accession no.: O60566
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... BUB1B(701)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D23 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Mitotic checkpoint serine/threonine-protein kinase BUB1β (BubR1). It targets an epitope within 25 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the internal region of human BubR1.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BubR1 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BubR1 in lysates from HT-29 cells and Human testis tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BubR1 in Human testis tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected BubR1 in one million Jurkat cells.

Affinity Binding Assay: A representative lot of this antibody bound BubR1 peptide with a KD of 3.7 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (UniProt: O60566), also known as Mitotic MAD3/BUB1-related protein kinase or budding uninhibited by benzimidazole 1-related 1 (BUBR1). It is encoded by the BUB1B gene (Gene ID: 701) in human. BUBR1 is a serine/threonine protein kinase and a member of the BUB1 family. It consists of multiple functional domains, including a kinase domain responsible for catalytic activity as well as domains involved in binding to other mitotic checkpoint proteins and kinetochores. BUBR1 is an essential component of mitotic checkpoint complex (MCC). With other proteins it coordinates and monitor the attachment of chromosomes to the mitotic spindle and prevents premature cell division until all chromosomes are correctly aligned at the metaphase plate. BUBR1 also involved in regulating the activity of the anaphase-promoting complex/cyclosome (APC/C), a key regulator of cell cycle progression and degradation of specific proteins. Furthermore, BUBR1 contributes to DNA damage response pathways and mitotic exit control. BUBR1 expression is tightly regulated throughout the cell cycle, with peak levels during metaphase and decreased expression during anaphase and telophase in actively dividing cells during mitosis. It is particularly abundant in tissues with high cell turnover rates such as testes, thymus, and bone marrow. BUBR1 gene mutation or dysregulation can result in chromosomal instability, aneuploidy, and defective chromosome segregation, which are hallmarks of cancer development and progression. BUBR1 is downregulated with age and associated with age-related disorders. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bloom, CR., and North, BJ. (2021) Cell Biosci. 11(1);76, Elowe, S., and Bolanos-Garcia, VM. (2022) Trends Biochem. Sci., 47(4); 352-366)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable