ZRB2488

Anti-MR1 Antibody, clone 1D18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Major histocompatibility complex class I-related gene protein (UniProt: Q95460, also known as MHC class I-related gene protein, Class I histocompatibility antigen-like protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1D18 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 39.37 kDa; observed mol wt ~38 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Extracellular domain
• Protein ID accession no.: (NP_001372090 from blast but Uniprot lists NP_001522)
• UniProt accession no.: Q95460
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... MR1(3140)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects MHC class I-related gene protein (MR1). It targets an epitope within 23 amino acids in the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the extracellular domain of human MHC class I-related gene protein (MR1).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MR1 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected MR1 in lysates from HaCaT cells and 1:1,000 dilution in HepG2 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound MR1 peptide with a KD of 9.3 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MR1 in HaCaT cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Major histocompatibility complex class I-related gene protein (UniProt: Q95460; also known as MHC class I-related gene protein, Class I histocompatibility antigen-like protein) is encoded by the MR1 gene (Gene ID: 3140) in human. MR1 is a single-pass membrane glycoprotein that is ubiquitous in its distribution and can heterodimerize with β2-microglobulin (B2M). It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. The mature form has an extracellular domain (aa 23-302), a transmembrane domain (aa-303-323), and a short cytoplasmic tail (aa 324-341). Its ligand binding region is localized to amino acids 23-201. MR1 serves as an antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. It is involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain known as mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and may be involved in monitoring commensal flora or serve as a distress signal. MR1-restricted T cells can discriminate between microbes in a TCR-dependent manner. However, these cells are shown to be severely reduced in circulation in subjects with chronic HIV-1 infection. Five different isoforms of MR1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Young, MH et al., (2013). PLOS one 8(1); e53789; Leeansyah, E., et al. (2013). Blood. 121(7); 1124-1135; Kjer-Nielsen, L., et al. (2012). Nature. 491(7426); 717-723).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable