ZRB1528

Anti-GLTSCR1L Antibody, clone 1L13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): BRD4-interacting chromatin-remodeling complex-associated protein-like;Glioma tumor suppressor candidate region gene 1 protein-like
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1L13, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 115.08 kDa; observed mol wt ~110 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): nonhuman primates
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001305748
• UniProt accession no.: Q6AI39
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... BICRAL(23506)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1L13 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Glioma tumor suppressor candidate region gene 1 protein-like (GLTSCR1L). It targets an epitope within 24 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Glioma tumor suppressor candidate region gene 1 protein-like (GLTSCR1L).

Application

Quality Control Testing

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLTSCR1L in HCT116 cell lysate.

Tested applications

Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected GLTSCR1L in NIH3T3, A431, Raw264.7, and Jurkat cell lysates.

Western Blotting Analysis: A representative lot of this antibody detected GLTSCR1L in mononuclear primary cell cultures from bone marrow, spleen plus human PlatE cells, and LNCaP cells.

Flow Cytometry Analysis (FC): 1 μg from a representative lot detected GLTSCR1L in one million Jurkat cells.


Affinity Binding Assay: A representative lot of this antibody bound GLTSCR1L peptide with a KD of 3.2 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLTSCR1L in HCT116 cell lysate.

Target description

BRD4-interacting chromatin-remodeling complex-associated protein-like (UniProt: Q6AI39; also known as Glioma tumor suppressor candidate region gene 1 protein-like, GLTSCR1L) is encoded by the BICRAL (also known as GLTSCR1L, KIAA0240) gene (Gene ID: 23506) in human. GLTSCR1L is a component of the SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. The mammalian SWI/SNF complex is an ATP-dependent chromatin remodeler composed of 10 - 14 subunits and is implicated in a variety of processes including mitosis, DNA replication, DNA damage repair, genomic looping, and gene splicing, GLTSCR1L knockouts in the metastatic prostate cancer cell line PC3 is shown to result in a loss in proliferation and colony-forming ability. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Alpsoy., and Dykhuizen, EC. (2018). J. Biol. Chem. 293(11); 3892-3903).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable