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SRP5327

Sigma-Aldrich

BUB1B, active, GST tagged human

PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO®

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

1.2-1.8 nmol/min·mg

mol wt

~73 kDa

NCBI accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... BUB1B(701)

General description

BUB1B or budding uninhibited by benzimidazoles 1 homolog beta is involved in spindle checkpoint function and has been localized to the kinetochore. BUB1B plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. BUB1B links the regulation of chromosome-spindle attachment to mitotic checkpoint signaling. BUB1B expression during the spindle checkpoint is dependent on residual BUB1B expression and impaired spindle checkpoint function has been found in many forms of cancer. BUB1B is essential for early embryonic development and normal hematopoiesis.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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