SRP2119
RNA Polymerase II, RPB12 subunit, GST tagged human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.0, hRPB7.0, hsRPB10a
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
frozen liquid
mol wt
~33 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
100 μg/mL
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... POLR2K(5440)
General description
POLR2K (RNA polymerase II subunit K) is located on human chromosome 8q22. Rpb12 is the eukaryotic subunit that is also called as ABC10α. Rpb12 is the general subunit of all three eukaryotic RNA polymerases.
Biochem/physiol Actions
RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability. RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart.
RPB12/RPC10 (RNA polymerase II subunit K) is required for growth in Saccharomyces cerevisiae. The carboxy-terminal domain (CTD) of RNAP II plays a major role in recruiting many of these activities to the transcriptional machinery.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Woychik, N.A., et al.
Transcription Mechanisms and Regulation, 227-242 (1994)
N A Woychik et al.
The Journal of biological chemistry, 265(29), 17816-17819 (1990-10-15)
The Saccharomyces cerevisiae gene encoding the smallest RNA polymerase II subunit, RPB10, was isolated and sequenced. The gene for this subunit is present in single copy and maps to chromosome XV, where two other yeast RNA polymerase II subunits, RPB2
RNA polymerase II conducts a symphony of pre-mRNA processing activities.
Howe K J.
Biochimica et Biophysica Acta, 1577(2), 308-324 (2002)
The archaeal RNA polymerase subunit P and the eukaryotic polymerase subunit Rpb12 are interchangeable in vivo and in vitro.
Reich C, et al.
Molecular Microbiology, 71(4), 989-1002 (2009)
Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors.
Heidenblad M, et al.
BMC Medical Genomics, 1(1), 3-3 (2008)
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