biological source
Plasmodium falciparum
recombinant
expressed in baculovirus infected insect cells
assay
≥40% (SDS-PAGE)
form
aqueous solution
mol wt
55 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
Plasmodium falciparum ... HDAC1(813532)
General description
Plasmodium falciparum HDAC1, GenBank Accession No. XM_001352091, full length with C-terminal HIS6-DDDDK (FLAG) tags, MW = 55 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
Physical form
Formulated in 50 mM Tris-HCl, pH 8.0, 130 mM NaCl and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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