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SAE0215

Sigma-Aldrich

Inorganic Pyrophosphatase from Escherichia coli

≥100 units/mL, buffered aqueous solution

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About This Item

UNSPSC Code:
12352204

Pricing and availability is not currently available.

biological source

Escherichia coli

Quality Level

recombinant

expressed in E. coli

grade

for molecular biology

description

Recombinant, expressed in E.coli

assay

≥95% (size exclusion chromatography)

form

buffered aqueous solution

specific activity

≥100 units/mL

shelf life

2 yr at -20 °C ((retest))

mol wt

19.7 kDa

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Quality Level

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General description

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi)[1][2].

Application

This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunit[3]. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable protein[4].

Biochem/physiol Actions

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate to phosphate.[5] It plays an important role in energy metabolism as it provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis.[6]

Features and Benefits

This product has a purity minimum of 95% (SEC-HPLC) and an activity minimum of 100 units/mL to enhance RNA yield during transcription.

Unit Definition

One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25 °C. The reaction buffer used for determination of enzyme activity contains 50 mM Tris-HCl, pH 9.0.

Physical form

The product is supplied as an aqueous solution containing 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% glycerol, titrated to pH 8 at 25 °C.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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