SAB1309056

[KO Validated]Anti-LIN28B antibody produced in mouse

[Knockout Validated]purified monoclonal antibody, buffered aqueous solution

• Synonym(s): Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2
• UNSPSC Code: 12352203

Properties

• biological source: (Human)
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: monoclonal
• form: liquid
• mol wt: 27084Da
• species reactivity: human
• technique(s): western blot: 1:500-1:2000
• isotype: IgG1, kappa
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified
• Gene Information: human ... LIN28B(389421)

Description

General description

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5′-GGAG-3′ motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5′-GGAG-3′ motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Specificity

This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.

Physical form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable