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R4132

Sigma-Aldrich

Reticulocyte Stain

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5 MG
SGD 164.00

SGD 164.00

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5 MG
SGD 164.00

About This Item

UNSPSC Code:
41116121
NACRES:
NA.47

SGD 164.00

Please contact Customer Service for Availability
Request a Bulk Order

form

solution

Quality Level

500

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

application(s)

hematology
histology

storage temp.

room temp

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1 of 4

This Item
R5250R5000R5125
Ribonuclease A from bovine pancreas 4×cryst., ~70 U/mg (acc. to Kunitz)

83834

Ribonuclease A from bovine pancreas

Ribonuclease A from bovine pancreas Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5250

Ribonuclease A from bovine pancreas

Ribonuclease A from bovine pancreas Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R5000

Ribonuclease A from bovine pancreas

Ribonuclease A from bovine pancreas Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R5125

Ribonuclease A from bovine pancreas

form

powder

form

buffered aqueous solution

form

solid

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

mol wt

~13,700, Mr ~13700

mol wt

~13,700

mol wt

~13,700

mol wt

~13,700

impurities

proteases, none detected

impurities

-

impurities

salt, essentially free

impurities

salt, essentially free

Application

Utilizes New Methylene Blue for the identification of reticulocytes in blood films. Reticulocyte Stain has been used in the detection of reticulocytes.[1]

Principle

Blood and reticulocyte staining solution (New Methylene Blue) are mixed and incubated briefly at room temperature. Wedge or spun smears are made on microscope slides, air dried and evaluated under oil immersion on a light microscope. A red blood cell scoring positive would be observed containing two or more blue-stained granules.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCJ2295
    SLCG1782
    SLCB9528
    SLBZ7297
    SLBZ3283

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    HDL cholesterol levels are an important factor for determining the lifespan of erythrocytes
    Meurs I, et al.
    Experimental Hematology, 33(11), 1309-1319 (2005)
    Thiet M Vu et al.
    Nature, 550(7677), 524-528 (2017-10-19)
    Sphingosine-1-phosphate (S1P), a potent signalling lipid secreted by red blood cells and platelets, plays numerous biologically significant roles. However, the identity of its long-sought exporter is enigmatic. Here we show that the major facilitator superfamily transporter 2b (Mfsd2b), an orphan

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