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L9125

Sigma-Aldrich

L-Leucine-p-nitroanilide

leucine aminopeptidase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

L-Leucine-4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C12H17N3O3
CAS Number:
4178-93-2
Molecular Weight:
251.28
Beilstein/REAXYS Number:
2218433
EC Number:
224-047-3
MDL number:
MFCD00063681
UNSPSC Code:
12352204
PubChem Substance ID:
24896516
NACRES:
NA.32

Product Name

L-Leucine-p-nitroanilide, leucine aminopeptidase substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

ethanol: 50 mg/mL

storage temp.

2-8°C

SMILES string

CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C12H17N3O3/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18/h3-6,8,11H,7,13H2,1-2H3,(H,14,16)/t11-/m0/s1

InChI key

AXZJHDNQDSVIDR-NSHDSACASA-N

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Application

L-Leucine-p-nitroanilide has been used as substrate
  • for the determination of intestinal leucine aminopeptidase activity of juvenile rainbow trout (Oncorhynchus mykiss)
  • in insulin-regulated aminopeptidase inhibition assay
  • for determining exopeptidase activity

Substrates

Substrate for the colorimetric determination of leucine aminopeptidase.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ2165
SLBZ4570
SLBV6556
SLBQ6037V
SLBM6431V

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Identification of drug-like inhibitors of insulin-regulated aminopeptidase through small-molecule screening
Engen K, et al.
Assay and Drug Development Technologies, 14(3), 180-193 (2016)
W Bawab et al.
The Biochemical journal, 286 ( Pt 3), 967-975 (1992-09-15)
Aminopeptidase activities were identified in extracts of kidney, ovotestis, head ganglia, heart and haemolymph of Aplysia californica. These enzyme preparations hydrolysed [3H][Leu]enkephalin at the Try-1-Gly-2 bond as determined by h.p.l.c. analysis of cleavage products. In all these tissues, enkephalin-degrading aminopeptidase
K W Garren et al.
Pharmaceutical research, 6(11), 966-970 (1989-11-01)
The simultaneous diffusion and metabolism of the D- and L-isomers of the aminopeptidase substrate, leucine-p-nitroanilide (LPNA), were examined in vitro in the hamster cheek pouch. L-LPNA was completely hydrolyzed during diffusion across the cheek pouch, whereas D-LPNA crossed the cheek
Effects of fish meal replacement with meat and bone meal using garlic (Allium sativum) powder on growth, feeding, digestive enzymes and apparent digestibility of nutrients and fatty acids in juvenile rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
Esmaeili M, et al.
Aquaculture Nutrition, 23(6), 1225-1234 (2017)
I M Cesari et al.
The Journal of parasitology, 69(2), 280-284 (1983-04-01)
An aminopeptidase activity capable of hydrolysing leucine 4-nitroanilide and alanine 4-nitroanilide at pH 7.0 was detected in saponin-CaCl2 extracts and homogenates of adult Schistosoma mansoni. The extracts were also capable of acting on synthetic dipeptides at the same pH, preferentially
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