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Key Documents

I2660

Sigma-Aldrich

IRAK2, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

MG C150550

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

5-7 nmol/min·mg

mol wt

~103 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... IRAK2(3656)

Related Categories

Biochem/physiol Actions

IRAK2 (interleukin-1 receptor-associated kinase 2) belongs to the IRAK family and is ubiquitously expressed. The protein contains an amino terminal death domain and a central kinase domain. IRAK2 is required for TLR (Toll-like receptor) signaling. Upon activation of TLR4 and TLR8, IRAK2 is crucial for the induction of TNF (tumor necrosis factor). It also regulates endoplasmic reticulum stress signaling.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Samir Benosman et al.
PloS one, 8(5), e64256-e64256 (2013-06-01)
Endoplasmic reticulum (ER) stress occurs when unfolded proteins accumulate in the lumen of the organelle, triggering signal transduction events that contribute either to cellular adaptation and recovery or alternatively to cellular dysfunction and death. ER stress has been implicated in
Weina Zhang et al.
The Journal of biological chemistry, 289(18), 12507-12519 (2014-03-26)
The IL-1 receptor-associated kinases (IRAKs) are key regulators of Toll-like receptor (TLR)/IL-1 signaling, which are critical regulators of mammalian inflammation and innate immune response. Single nucleotide polymorphisms (SNPs) within the IRAK genes have been discovered recently. However, the functions of
H Wesche et al.
The Journal of biological chemistry, 274(27), 19403-19410 (1999-06-26)
The interleukin-1 receptor-associated kinase (IRAK) was first described as a signal transducer for interleukin-1 (IL-1) and has later been implicated in signal transduction of other members of the Toll/IL-1 receptor family. We now report the identification and characterization of a
Hui Wang et al.
The Journal of biological chemistry, 289(33), 23123-23131 (2014-06-29)
Within innate immune signaling pathways, interleukin-1 receptor-associated kinases (IRAKs) fulfill key roles downstream of multiple Toll-like receptors and the interleukin-1 receptor. Although human IRAK4 deficiency was shown to lead to severe immunodeficiency in response to pyogenic bacterial infection during childhood
M Muzio et al.
Science (New York, N.Y.), 278(5343), 1612-1615 (1997-12-31)
The interleukin-1 receptor (IL-1R) signaling pathway leads to nuclear factor kappa B (NF-kappaB) activation in mammals and is similar to the Toll pathway in Drosophila: the IL-1R-associated kinase (IRAK) is homologous to Pelle. Two additional proximal mediators were identified that

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