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D2321

Sigma-Aldrich

DAF-FM DA solution

5 mM in DMSO, ≥97% (HPLC)

Synonym(s):

4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate

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About This Item

Empirical Formula (Hill Notation):
C25H18F2N2O7
CAS Number:
254109-22-3
Molecular Weight:
496.42
MDL number:
MFCD16872050
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

assay

≥97% (HPLC)

form

DMSO solution

storage condition

desiccated
protect from light

color

clear colorless

shipped in

wet ice

storage temp.

−20°C

SMILES string

Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

Biochem/physiol Actions

DAF-FM DA is a cell permeable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Other Notes

Light sensitive

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
126M4723V
086M4738V
035M4721V
104M4717V
083M4741V

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Aurora Parlati et al.
Frontiers in plant science, 8, 1299-1299 (2017-08-10)
The PII protein in plants has been associated to many different tissue specialized roles concerning the Nitrogen assimilation pathways. We report here the further characterization of
Diana Passaro et al.
Cancer cell, 32(3), 324-341 (2017-09-06)
The biological and clinical behaviors of hematological malignancies can be influenced by the active crosstalk with an altered bone marrow (BM) microenvironment. In the present study, we provide a detailed picture of the BM vasculature in acute myeloid leukemia using
Gen Li et al.
Frontiers in plant science, 11, 1215-1215 (2020-09-10)
Anthocyanin-derived fleshy fruit pigmentation has become an excellent system for studying the regulatory network underlying fruit ripening and quality. The transcriptional control of anthocyanin biosynthesis by MYB-bHLH-WDR complexes has been well established, but the intermediate signals through which the environmental
Caihua Wang et al.
Stem cell research & therapy, 10(1), 175-175 (2019-06-15)
Cancer stem cells (CSCs) play a critical role in tumor development and progression and are involved in cancer metastasis. The role of reactive oxygen species (ROS) in CSCs and cancer metastasis remains controversial. The aim of the present study was
Diego Piacentini et al.
Environment international, 132, 105094-105094 (2019-09-05)
Over the last years, various acellular assays have been used for the evaluation of the oxidative potential (OP) of particular matter (PM) to predict PM capacity to generate reactive oxygen (ROS) and nitrogen (RNS) species in biological systems. However, relationships

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