LLCMK2 (Original)

85062804, rhesus monkey kidney, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: LLCMK2 (Original), 85062804, from rhesus monkey kidney, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85062804, rhesus monkey kidney, Epithelial, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• biological source: rhesus monkey kidney
• packaging: tube of 5 μg 85062804-DNA-5UG; pkg of vial of cells 85062804-1VL
• growth mode: Adherent
• karyotype: 2n = 42
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Monkey Rhesus kidney

Cell Line Description

Susceptible to a wide range of viruses including enterovirus, rhinovirus, myxovirus and poxvirus groups.

Application

LLCMK2 (Original) has been used to infect and maintain Trypanosoma cruzi parasite culture and as a medium for Sendai virus titer determination. It has also been used for viral culture.

Culture Medium

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 5% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.