TK6 (IVGT)

13051501, human spleen, Small round cells in suspension

• UNSPSC Code: 41106514

Properties

• Product Name: TK6 (IVGT), 13051501, from human spleen
• biological source: human spleen
• description: Human lymphoblast, thymidine kinase heterozygote, mutagenesis assay, diploid human
• packaging: tube of 5 μg 13051501-DNA-5UG; pkg of vial of cells 13051501-1VL
• growth mode: Suspension
• morphology: Small round cells in suspension
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

JCRBNo 1435, lot number 11032011. This line is a derivative of the WIL-2 lymphoblast cell line. The cells are heterozygous at the thymidine (TK) kinase locus and can be used to quantitatively detect forward mutations at 3 loci.

Culture Medium

RPMI 1640 (R0883) + 10% Horse Serum + 2mM L-Glutamine (G7513) + 1.8 mMolar Sodium Pyruvate(NaP) (S8636). Cryopreserve cells in culture medium with cells heat-inactivated 10% Horse Serum and 10% Dimethyl sulphoxide (DMSO)

Subculture Routine

Dilute cell in fresh medium at 0.1- 1.5 x 10⁵ cells per ml.

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

If use of this culture results in a scientific publication, it should be cited in the publication as: TK6 (IVGT) (ECACC 13051501)