LIM2405 cell line human

12062003, human colon, epithelial

• Synonym(s): LIM 2405, LIM-2405
• UNSPSC Code: 41106514

Properties

• Product Name: LIM2405 cell line human, 12062003, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 12062003, human colon, epithelial
• biological source: human colon
• packaging: tube of 5 μg 12062003-DNA-5UG; pkg of vial of cells 12062003-1VL
• growth mode: adherent
• morphology: epithelial
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Human adenocarcinoma of the caecum of a male patient. Cell Bank Australia reference number: CBA0165.

Culture Medium

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM

Subculture Routine

Optimal split ratio 1:4-1:6 (seeding density 1.6 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM2405 may take 2-3 days to seed after thawing or trypsinisation.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.