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Key Documents

LIM1899 cell line human

12062001, human colon, Epithelial; cells are small rounded and grow in clusters

Synonym(s):

LIM 1899, LIM-1899

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About This Item

UNSPSC Code:
41106514

Product Name

LIM1899 cell line human, 12062001

biological source

human colon

packaging

tube of 5 μg 12062001-DNA-5UG
pkg of vial of cells 12062001-1VL

growth mode

adherent

morphology

Epithelial; cells are small rounded and grow in clusters

shipped in

dry ice

storage temp.

−196°C

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Cell Line Description

Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.

Culture Medium

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM

Subculture Routine

Optimal split ratio 1:8 (seeding density 1 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.

Other Notes

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