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C-20211

Sigma-Aldrich

Keratinocyte Growth Medium 2

Basal Medium, 500 ml

Synonym(s):

Recommended with C-39016 or C-39011

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.

form

aqueous solution

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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This Item
C-20216C-39011C-20111
Keratinocyte Growth Medium 2 Basal Medium, 500 ml

C-20211

Keratinocyte Growth Medium 2

Keratinocyte Growth Medium 2 Basal Medium, phenol red-free, 500 ml

C-20216

Keratinocyte Growth Medium 2

Keratinocyte Growth Medium 2 SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

C-39011

Keratinocyte Growth Medium 2

Keratinocyte Growth Medium 2 Kit including Basal Medium and SupplementPack, 500 ml

C-20111

Keratinocyte Growth Medium 2

form

aqueous solution

form

aqueous solution

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

packaging

pkg of 500 ml

packaging

pkg of 500 ml

packaging

pkg of 1 kit

packaging

pkg of 500 ml

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

General description

Keratinocyte Basal Medium 2 (supplements sold separately)

To form a complete medium, it should be combined with the media supplements: C-39016 or C-39011

Application

Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells. The medium is optimized for primary human cells but can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.Recommended for:Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskinNormal Human Epidermal Keratinocytes (NHEK), adult donor

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Keratinocyte Growth Medium 2 are subjected to comprehensive quality control tests using primary human keratinocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested keratinocytes. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Subculture Routine

Click here for more information.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Articles

    Primary Human Epidermal Keratinocytes Cell Culture System

    Primary human keratinocytes and serum-free keratinocyte growth media for the feeder cell-free in vitro cultivation of epidermal keratinocytes. Protocols for handling, cell passaging, media, and product use.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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