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Key Documents

PC709

Sigma-Aldrich

Anti-MYH (33-51) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-MutY Homolog

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MUTYH(4595)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.
Recognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.
This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).

Immunogen

Human
a synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH

Application

Immunoblotting (1:1500)

Immunofluorescence (1:500)

Paraffin Sections (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
PC12, Jurkat, or HeLa cells

Other Notes

Detects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
Wang, G., et al. 2000. Nucleic Acids Res.28, 2135.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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G Wang et al.
Nucleic acids research, 28(10), 2135-2140 (2000-04-25)
Generation of reactive oxygen species (ROS) and activation of a transcriptional program that mimics the hypoxic response have been documented in cultured cells in the presence of cobalt chloride. We found that in the presence of hypoxia-mimicking concentrations of CoCl(2)

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