antibody form
ascites fluid
Quality Level
clone
2B7
monoclonal
purified by
(unpurified)
species reactivity
mouse, rat, human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
General description
Purified recombinant fragment of human PRKAA1 expressed in E. Coli.
Application
Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in ovarian cancer and brain tissues.
Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in Ntera-2 cells.
Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected PRKAA1 in PC-2 cells.
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in Ntera-2 cells.
Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected PRKAA1 in PC-2 cells.
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Quality
Evaluated by Western Blotting in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.
Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKAA1 in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.
Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKAA1 in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.
Physical properties
~65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Mouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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