MABN2301
Anti-NLGN4X Antibody, clone CSTEM30
Synonym(s):
Neuroligin-4 X-linked;Neuroligin X;HNLX
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
CSTEM30, monoclonal
mol wt
calculated mol wt 91.92 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG2aκ
epitope sequence
Extracellular domain
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
human ... NLGN4X(57502)
General description
Neuroligin-4, X-linked (UniProt: Q8N0W4; also known as Neuroligin X, HNLX) is encoded by the NLGN4X (also known as KIAA1260, NLGN4, UNQ365/PRO701) gene (Gene ID: 57502) in human. NLGN4X is a single-pass type I membrane, homodimeric, glycoprotein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 42-676) a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). It is predominantly expressed in the heart tissue. It is also expressed at lower levels in the liver, skeletal muscle, pancreas, and brain. It is a putative neuronal cell surface protein that is involved in cell-cell-interactions. In human, NLGN4X is localized at excitatory synapses, whereas in mouse NLGN4-like is localized at glycinergic synapses. NLGN4X can be phosphorylated by protein kinase C that enhances excitatory synapses. Mutations in NLGN4X gene have been linked to X-linked Asperger syndrome and Autism. (Ref.: Nguyen, TA., et al. (2020). Front. Synaptic Neurosci. 12; 33; Laumonnier, F., et al. (2004). Am. J. Hum. Genet. 74(3); 552-557).
Specificity
Clone CSTEM30 is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X). It targets an epitope within the extracellular domain.
Immunogen
His-tagged recombinant protein fragment corresponding to the extracellular domain of human Neuroligin-4,x-linked (NLGN4X).
Application
Quality Control Testing
Evaluated by Flow Cytometry in HFF-hiPSCs.
Flow Cytometry Analysis (FC): 1 μg of this antibody detected NLGN4X in one million Human foreskin fibroblast-induced pluripotent stem cells (HFF-hiPSCs) .
Tested applications
Flow Cytometry Analysis: A representative lot detected NLGN4X in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640; Liu, X., et al. (2017). Nat Methods. 14(11):1055-1062; Liu, X., et al. (2020). Nature. 586(7827):101-107).
Immunocytochemistry Analysis: A representative lot detected NLGN4X in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).
ELISA Analysis: A representative lot detected NLGN4X in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Flow Cytometry in HFF-hiPSCs.
Flow Cytometry Analysis (FC): 1 μg of this antibody detected NLGN4X in one million Human foreskin fibroblast-induced pluripotent stem cells (HFF-hiPSCs) .
Tested applications
Flow Cytometry Analysis: A representative lot detected NLGN4X in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640; Liu, X., et al. (2017). Nat Methods. 14(11):1055-1062; Liu, X., et al. (2020). Nature. 586(7827):101-107).
Immunocytochemistry Analysis: A representative lot detected NLGN4X in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).
ELISA Analysis: A representative lot detected NLGN4X in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NLGN4X, clone CSTEM30, Cat. No. MABN2301, is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X) and is tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.
Physical form
Purified mouse monoclonal antibody Ig G2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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