MABN1615
Anti-Cdh1 Antibody, clone 15A9.1
clone 15A9.1, from mouse
Synonym(s):
Fizzy-related protein homolog, CDC20-like protein 1, CDH1alpha, CDH1beta, Cdh1/Hct1 homolog, hCDH1, Fzr, Fzr1, Fzr2
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
15A9.1, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... FZR1(51343)
General description
Fizzy-related protein homolog (UniProt Q9UM11; also known as CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, Fzr) is encoded by the FZR1 (also known as CDH1, FYR, FZR, KIAA1242) gene (Gene ID 51343) in human. The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase involved in cell cycle regulation. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. DNA-end resection factor CtIP. Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while Cdc14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to Polo-like kinase 1 (Plk1) degradation. Following DNA double-strand breaks (DSB) repair, Cdk1 and Plk1 are reactivated to allow cell cycle progression from G2 into mitosis. Cdh1-depleted cells or Cdh1 / mice exhibit elevated levels of DNA damage and chromosomal aberrations. APC/C-Cdh1 interacts with the KEN box motif on its target proteins and known APC/C-Cdh1 substrates include the the de-SUMOylation enzyme SENP2, and and the fragile X syndrome protein FMRP.
Specificity
Clone 15A9.1 targeted epitope lies within the N-terminal region of mouse and rat Cdh1, as well as all three human Cdh1 spliced isoforms reported by UniProt (Q9R1K5, B1WCA1, and Q9UM11).
Immunogen
GST-tagged recombinant human Cdh1 N-terminal fragment.
Application
Anti-Cdh1, clone 15A9.1, Cat. No. MABN1615, is a highly specific mouse monoclonal antibody that targets Cdh1 and has been tested in Western Blotting.
Research Category
Neuroscience
Neuroscience
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Cdh1 in 10 µg of embryonic (E13-14 & E16) and neonatal (P1 & P7) mouse brain tissue lysates, as well as in mouse embryonic stem cell (mESC) and neural stem cell (mNSC) lysates.
Quality
Evaluated by Western Blotting in mouse E16 brain tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cdh1 in 10 µg of embryonic E16 mouse brain tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cdh1 in 10 µg of embryonic E16 mouse brain tissue lysate.
Target description
~55 kDa observed. 55.18 kDa (human isoform 1), 54.77 (human isoform 2, CDH1alpha or Fzr1), 44.87 kDa (human isoform 3, CDH1beta or Fzr2), and 54.69 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service