MABF3098

Anti-phospho-CARD11 (Ser893) Antibody, clone 25B10

• Synonym(s): Carma 1, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 25B10, monoclonal
• mol wt: calculated mol wt 133.28 kDa; observed mol wt ~150 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001311210
• UniProt accession no.: Q9BXL7
• storage temp.: 2-8°C
• Gene Information: human ... CARD11(84433)

Description

Specificity

Clone 25B10 is a mouse monoclonal antibody that detects phospho-CARD11 (Ser893). It targets an epitope from the C-terminal half.

Immunogen

Ovalbumin-conjugated linear peptide surrounding phosphoserine 893 from the C-terminal half of human CARD11.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from Jurkat cells treated with PMA and Ionomycin.

Western Blotting Analysis (WB): A 1:500 Dilution of this antibody detected phospho-CARD11 (Ser893) in lysates from Jurkat cells treated with Phorbol 12-myristate 13-acetate (PMA; 15 ng/mL) and Ionomycin (1 g/mL) for 20 minutes.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected phospho-CARD11 (Ser893) in lysates from Jurkat cells treated with Phorbol 12-myristate 13-acetate (PMA; 15 ng/mL) and Ionomycin (1 g/mL) for 20 minutes.

Immunoprecipitation Analysis: A representative lot detected phospho-CARD11 (Ser893) in Immunoprecipitation applications (Kutzner, K., et al. (2022). Sci Signal. 15(723):eabk3083).

Western Blotting Analysis: A representative lot detected phospho-CARD11 (Ser893) in Western Blotting applications (Kutzner, K., et al. (2022). Sci Signal. 15(723):eabk3083).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Caspase recruitment domain-containing protein 11 (UniProt: Q9BXL7; also known as CARD-containing MAGUK protein 1, Carma 1) is encoded by the CARD11 (also known as CARMA1) gene (Gene ID: 84433) in human. CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, characterized by the presence of a caspase activation and recruitment domain (CARD) (aa 18-110), a coiled-coil domain (aa 130-449), a membrane-binding domain (PDZ) (aa 667-755) and a guanylate kinase-like domain (aa 973-1140). These structural features enable CARD11 to function as a scaffold protein, facilitating the assembly of signaling complexes in immune cells. The protein undergoes post-translational modifications, including phosphorylation, that promotes the assembly of the CARD11-BCL10-MALT1 (CBM) complex and lymphocyte activation. Phosphorylation can also suppress CARD11 functions in T or B cells. There are several constitutive and inducible CARD11 phosphorylation sites in T cells. Phosphorylation of Ser893 of CARD11 prevents the activation of the transcription factor NF- B, the kinase JNK, and the protease MALT1. Mutations of the CARD11 gene or dysregulation of CBM complex signaling causes combined immune deficiency and can lead to uncontrolled cell proliferation and survival, contributing to tumorigenesis. Additionally, CARD11 is associated with autoimmune diseases due to its critical role in regulating immune responses. (Ref: Kutzner, K., et al. (2022). Sci Signal. 15(723):eabk3083)

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.