MABF3086
Anti-G-CSF-R/CD114 Antibody, clone LMM741
Synonym(s):
CD114, G-CSF receptor, G-CSF-R, Granulocyte colony-stimulating factor receptor
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
LMM741, monoclonal
mol wt
calculated mol wt 92.16 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
epitope sequence
Extracellular domain
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... CSF3R(1441)
Specificity
Clone LMM741 is a mouse monoclonal antibody that specifically detects G-CSF-R/CD114. It targets an epitope within the extracellular domain.
Immunogen
CHO-6A11 cells transfected with full length human G-CSF-R/CD114.
Application
Quality Control Testing
Evaluated by Flow Cytometry in human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis(FC): 1.0 ug from a representative lot detected G-CSF-R/CD114 in human peripheral blood mononuclear cells (PBMC).
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated G-CSF-R/CD114 in immunoprecipitation application (Nicholson, S.E., et al. (1994). Proc Natl Acad Sci USA. 91(8):2985-8).
Flow Cytometry Analysis: A representative lot detected G-CSF-R/CD114 in flow cytometry application (Layton, J.E., et al. (1999). J Biol Chem. 274(25):17445-51).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Flow Cytometry in human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis(FC): 1.0 ug from a representative lot detected G-CSF-R/CD114 in human peripheral blood mononuclear cells (PBMC).
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated G-CSF-R/CD114 in immunoprecipitation application (Nicholson, S.E., et al. (1994). Proc Natl Acad Sci USA. 91(8):2985-8).
Flow Cytometry Analysis: A representative lot detected G-CSF-R/CD114 in flow cytometry application (Layton, J.E., et al. (1999). J Biol Chem. 274(25):17445-51).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Granulocyte colony-stimulating factor receptor (UniProt: Q99062; also known as G-CSF receptor, G-CSF-R, CD114) is encoded by the CSF3R (also known as CSF3R) gene (Gene ID: 1441) in human. CD114/G-CSF-R belongs to the type I subgroup of the superfamily of cytokine receptors. G-CSF-R mediates granulocyte proliferation and differentiation in both normal and malignant hematopoiesis. G-CSF-R functions as the receptor for granulocyte colony-stimulating factor (G-CSF) and is activated via ligand-induced homodimerization. G-CSF-R is a single-pass type I membrane protein produced with a signal peptide sequence (aa 1-24), the removal of which yields the mature single transmembrane (aa 628-650) receptor with a C-terminal cytoplasmic tail (aa 651-836) and a large N-terminal extracellular domain (aa 25-627) composed of a C2-type Ig-like domain (aa 25-117) and five type-III fibronectin domains (aa 125-623). It is highly expressed in bone marrow cells, placenta, lung, lymphoid tissue and peripheral blood granulocytes. The G-CSF/GCSF-R complex mediates survival, proliferation, and maturation of neutrophils and regulates granulopoiesis under both normal and stress conditions. Cell signaling via G-CSF-R is transduced primarily via the Jak/STAT pathway, but the Ras/Raf/MAP kinase and PKB/Akt pathways are also involved. CSF3R gene mutations are well documented in congenital neutropenia as well as the associated predisposition to myelodysplasia and acute myeloid leukemia myeloproliferative disorders. (Ref.: Nicholson, S.E., et al. (1994). Proc Natl Acad Sci USA. 91(8):2985-8; Layton, J.E., et al. (1999). J Biol Chem. 274(25):17445-51).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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