MABF2122

Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1

clone PC 81-1, from mouse

• Synonym(s): Poly(rC)-binding protein 2, Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PC 81-1, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001122383
• UniProt accession no.: Q15366
• target post-translational modification: unmodified
• Gene Information: human ... PCBP2(5094)

Description

General description

Poly(rC)-binding protein 2 (UniProt: Q15366; also known as Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2) is encoded by the PCBP2 gene (Gene ID: 5094) in human. PCBP2 belongs to a class of cellular proteins that bind to poly(C) stretches of both RNA and DNA and has been detected in all tissues studied. PCBP2 shuttles between the nucleus and cytoplasm and exerts its impact on RNA processing and mRNA expression through sequence-specific interactions with C-rich determinants within target mRNAs. It acts as an important mediator of multiple processes, including replication of viruses with gastrointestinal and hepatic tropism, hepatic collagen synthesis, globin expression, and cellular proliferation. PCBP2 is shown to negatively regulate cellular antiviral responses mediated by mitochondrial antiviral-signaling protein (MAVS) signaling and acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, thereby triggering MAVS ubiquitination and degradation. Together with PCBP1, it is shown to function as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP2 contains three K-homologous (KH) domains that mediates poly(C) binding. Eight different isoforms of PCBP2 have been described that are produced by alternative splicing. (Ref.: Ghanem, LR et al. (2014). Gene Exp. Patterns 14(2); 78-87; Sean, P., et al. (2008). Virology. Virology. 378(2); 243-253).

Specificity

Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.

Immunogen

His-tagged full-length human recombinant Poly(rC)-binding protein 2.

Application

Anti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting.

Research Category
Inflammation & Immunology

Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).

Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).

Western Blotting Analysis: 1 µg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected PCBP2 (hnRNP E2) in HeLa cell lysate.

Target description

~38 kDa observed; 38.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.