MABC1235

Anti-N-Myc Interactor Antibody, clone 16E9.2

clone 16E9.2, from mouse

• Synonym(s): N-myc-interactor, N-myc and STAT interactor, Nmi
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 16E9.2, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_004679
• UniProt accession no.: Q13287
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... NMI(9111)

Description

General description

N-myc-interactor (UniProt Q13287; also known as N-myc and STAT interactor, Nmi) is encoded by the NMI gene (Gene ID 9111) in human. Nmi is expressed in all adult and fetal tissues except brain and skin and is shown to be more abundant in fetal liver. It interacts with all STATs, except STAT2 and augments STAT-mediated transcription in response to IL-2 and gamma-interferon. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, it enhances the association of CBP/p300 coactivator proteins with STAT1 and STAT5. Higher expression of Nmi has been reported in myeloid leukemia that also express higher levels of c-Myc. Ref.: Zhu, M., et al. (1999). Cell 96, 121-130.

Specificity

Clone 16E9.2 targeted epitope resides within the N-terminal half of N-Myc Interactor.

Immunogen

GST-tagged recombinant human N-Myc Interactor N-terminal fragment.

Application

Detect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Myc Interactor in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected N-Myc Interactor in human cerebellum, small instestine, and tonsil tissue sections.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-Myc Interactor in 10 µg of K562 cell lysate.

Target description

~39 kDa observed. 35.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1