GF06

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3)

liquid, clone 134A-2B3, Calbiochem^®

• Synonym(s): Anti-Transforming Growth Factor-α
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 134A-2B3, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, human
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TGFA(7039)

Description

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa denatured and native forms of TGFα protein.

Recognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.

This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).

Immunogen

Human

recombinant, human TGF-α (~6 kDa mature form)

Application

Immunoblotting (1-5 µg/ml, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).

Analysis Note

Negative Control
NIH3T3 cells

Positive Control
A2058 or ST-FeSV FRE cells

Other Notes

Sorvillo, J.M., McCormack, E.S. 1990. Oncogene5, 377.
Coffey, R.J., et al. 1987. Nature328, 817.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.

This antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg